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. 2023 Aug 11;32(22):3153–3165. doi: 10.1093/hmg/ddad135

Figure 4.

Figure 4

Lack of TAZ disturbs NUP205 expression. (A) Immunoblot showing whole-cell expression levels of endogenous NUP205 in hsMPs transfected with siRNA YAP or TAZ compared with control for 48 h. densitometry analysis of NUP205 expression normalized to actin (n = 8–9). Data are reported as mean ± SEM. *Significant decrease by siRNA TAZ (unpaired t-test; P < 0.05). (B) Immunoblot showing endogenous expression levels of NUP205, TAZ, and GAPDH, in HEK293T cells transfected with siRNA TAZ compared with control, after 6, 12, 16, and 24 h. Representative blot for n = 3. Quantification of relative TAZ (dashed) and NUP205 (line) whole-cell expression normalized to GAPDH (n = 3). (C) qPCR shows decrease of relative mRNA expression of TAZ (dashed) and NUP205 (line) in HEK293T cells transfected with siRNA TAZ compared with control (n = 3). Representative data from three independent experiments are shown. Data are reported as mean ± SEM. *Significant decrease by siRNA TAZ (unpaired t-test; P < 0.05).