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. 2023 Aug 11;32(22):3153–3165. doi: 10.1093/hmg/ddad135

Figure 5.

Figure 5

Lack of TAZ alters YAP localization. (A) Immunoblot showing downregulation of NUP205 and the nuclear-to-cytoplasmatic shift of YAP upon downregulation of TAZ after 16 h in HEK293T cell fractionation assay. Representative blot for n = 3. (B) Immunofluorescence staining of HEK293T cells transfected with siRNA TAZ for 16 h compared with control. TAZ downregulation on the left. Co-staining for endogenous NUP205, endogenous YAP, and nuclear stain with DAPI. Representative data from three independent experiments are shown. All scale bars = 20 μm. Summary of correlated total cell fluorescence of NUP205 (n = 81–95 cells) and summary of YAP nuclear/cytoplasmatic ratio quantification (n = 53–57 cells). Data are reported as mean ± SEM. *Significant decrease by siRNA TAZ (unpaired t-test; P < 0.05).