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. 2023 Nov 7;13:19263. doi: 10.1038/s41598-023-46496-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PS-PtychoLM imaging results of mouse eye (a–h) and heart tissue specimens (i–p). (a, i) Quantitative birefringence maps of the mouse eye and heart tissues over the entire FoV. (b, j) Quantitative phase images of the mouse eye and heart tissues over the entire FoV. (c–f) Enlarged birefringence and phase images of the parts marked with red and orange boxes in (a), respectively. (g, h) Hematoxylin and eosin (H&E) staining images of the parts (c, d) captured with an optical microscope (Nikon, ECLIPSE Ti, 0.2NA). (k–n) Enlarged birefringence and phase images of the parts marked with red and orange boxes in (i), respectively. (o, p) H&E staining images of the parts (k, l) captured with an optical microscope (Nikon, ECLIPSE Ti, 0.2NA). Overlaid white short lines on (c, d, k, and l) indicate the mean optic-axis orientation evaluated over a small region (70 μm × 70 μm).