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. 2023 Nov 7;13:19251. doi: 10.1038/s41598-023-46436-x

Figure 3.

Figure 3

Inhibitory effect of chaperonin 60 (Cpn60) on lipid accumulation in 3T3-L1 adipocytes. 3T3-L1 cells were differentiated using MDI and treated with various concentrations of Cpn60. (A) Lipid droplets in differentiated cells were visualized using Oil Red O staining (× 400 magnification). Scale bars indicate 0.5 mm. (B) Intracellular lipid content was quantified and compared with that in cells treated only with MDI. The data indicate the mean ± SD of values from three independent experiments. The p values were determined using ANOVA and Tukey’s honest significant difference test. Different letters indicate statistically significant differences (p < 0.05). NC, negative control; MDI, 1 μM dexamethasone, 0.5 mM IBMX, and 5 μg/mL insulin.