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. 2023 Nov 7;13:19251. doi: 10.1038/s41598-023-46436-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibitory effect of chaperonin 60 (Cpn60) on the expression of genes related to adipogenesis and lipogenesis in 3T3-L1 adipocytes. 3T3-L1 cells were cultured with MDI or MDI + 1 μg/mL Cpn60 and mRNA expression of genes was analyzed using qPCR. Fold change in the expression of adipogenic transcription factor (Pparg, Cebpa, and Srebf1) (A) and lipogenic transcription factor (Fas and Scd1) genes (B). The data indicate the mean ± SD of values from three independent experiments. The p values were determined using ANOVA and Tukey’s honest significant difference test. Different letters indicate statistically significant differences (p < 0.05). NC negative control, MDI, 1 μM dexamethasone, 0.5 mM IBMX, and 5 μg/mL insulin.