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. 2023 Nov 7;13:19251. doi: 10.1038/s41598-023-46436-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of chaperonin 60 (Cpn60) on lipid accumulation during different periods of treatment of 3T3-L1 cells with Cpn60. 3T3-L1 cells were differentiated by treatment with MDI or MDI + 1 μg/mL Cpn60 for different periods during differentiation (A). Lipid accumulation was quantified on day 8 by measuring lipid droplets stained with Oil Red O and comparing them with that in cells treated only with MDI (B). The data indicate the mean ± SD of values from three independent experiments. The p values were determined using ANOVA and Tukey’s honest significant difference test. Different letters indicate statistically significant differences (p < 0.05). NC, negative control; MDI, 1 μM dexamethasone, 0.5 mM IBMX, and 5 μg/mL insulin.