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. 2023 Nov 7;13:19251. doi: 10.1038/s41598-023-46436-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Binding of C/EBPβ on PPARγ or C/EBPα promoter and the expression of Gata2 and Gata3 investigated using chromatin immunoprecipitation (ChIP) assay. The cells were cultured with MDI or MDI + 1 μg/mL Cpn60 for 24 and 48 h and the binding of C/EBPβ on PPARγ or C/EBPα promoter was investigated using the ChIP assay (A). Fold change in the expression of Gata2 and Gata3 was analyzed using qPCR (B). The data indicate the mean ± SD of values from three independent experiments. The p values were determined using the Student’s t-test. Differences were considered significant at p < 0.05 (*) and p < 0.01 (**). MDI, 1 μM dexamethasone, 0.5 mM IBMX, and 5 μg/mL insulin.