TABLE 1.

Growth of unstarved and starved bacteria in Acanthamoeba buffer

Strain	Growth increment in Acanthamoeba buffera
	Unstarved bacteria	Starved bacteriab
M. avium serotype 4	72.2 ± 59.6	6.1 ± 6.3
L. pneumophila JR32	0.31 ± 0.13	0.72 ± 0.24
E. coli HB101	0.049 ± 0.067	NDc

^aNumber of CFU per milliliter after 10 days divided by initial number of CFU per milliliter (mean ± standard deviation from four independent experiments). The preparations were incubated at 33°C. 

^bStarved M. avium and L. pneumophila cells were incubated in nutrient-free buffer for 10 days to deplete the stored nutrients in the bacteria. 

^cND, not determined.