Figure 7:

Trial-by-trial response analysis to $SA{M}_{\mathrm{\Delta }100\text{\%}}$ to $SA{M}_{\mathrm{\Delta }25\text{\%}}$ with and without corticothalamic (CT) blockade. Single-units showing PPI changes larger than 0.3 at high $f_{mods}$ when switch from $SA{M}_{\mathrm{\Delta }100\text{\%}}$ to $SA{M}_{\mathrm{\Delta }25\text{\%}}$ are included in the trail by trial analysis. Group (n = 21) trial-by-trial responses to predictable SAM at $f_{mods}$ 128Hz (A) and 256Hz (B). These units show adapting responses to 10 presentations of repeating salient $SA{M}_{\mathrm{\Delta }100\text{\%}}$ stimuli (blue dot). Decreasing SAM modulation depth switched the trial-by-trial responses from adapting to predictable with spikes increasing with each successive presentation of the $SA{M}_{\mathrm{\Delta }25\text{\%}}$ stimulus (red dot). Optical CT blockade reversed the predictive response (green dot). Trend line slopes were significantly different for the three conditions for average spikes to predictable presentation of at $f_{mod}$ 128 Hz (A, ANCOVA, two-tailed, p < 0.05). Differences were significant at individual trial 7, 8, 9 and 10 in between $SA{M}_{\mathrm{\Delta }25\text{\%}}$ and $SA{M}_{\mathrm{\Delta }25\text{\%}}+CT$ stimulus conditions (p < 0.05, Friedman test followed Wilcoxon test) (A). Similarly, Trend line slopes were significantly different for the three conditions for average spikes to predictable presentation at $f_{mod}$ 256 Hz (B) (ANCOVA, two-tailed, p < 0.05). Differences were significantly different at trial 1, 3, 4, 5, 6, 7, 8, 9, and 10 between $SA{M}_{\mathrm{\Delta }25\text{\%}}$ vs. $SA{M}_{\mathrm{\Delta }25\text{\%}}$ with CT blockade. There were significant differences between $SA{M}_{\mathrm{\Delta }100\text{\%}}$ and $SA{M}_{\mathrm{\Delta }25\text{\%}}$ stimuli at trial 8 and 9 in their firing rates (B) (p < 0.05, Friedman test followed Wilcoxon test).