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. 2023 Nov 7;10(11):ENEURO.0125-23.2023. doi: 10.1523/ENEURO.0125-23.2023

Table 1.

List of antibodies used in this study

Antibody Concentration Species Antigen retrieval Source Catalog number RRID
(IHC) (WB)
Primary
 Anti-Arf4 1:200 1:1000 Rabbit polyclonal Hosoi et al. (2019)
 Anti-Arf5 1:200 1:1000 Rabbit polyclonal Hosoi et al. (2019)
 Anti-α-Tubulin 1:3000 Mouse monoclonal Sigma-Aldrich MABT205 AB_11204167
 Anti-BLBP 1 μg/ml Goat polyclonal Nittobo Medical Co Ltd. MSFR100290 AB_2571664
 Anti-BrdU 1 μg/ml Mouse monoclonal 2 N HCl, 37°C, 10 min BD Transduction Laboratories 3D4 AB_2033929
 Anti-Cux1 1 μg/ml Rabbit polyclonal CA (pH 6.0), 75°C, 1 h Santa Cruz Biotechnology sc-13024 AB_2261231
 Anti-EEA1 1 μg/ml Rabbit polyclonal Fukaya et al. (2014) AB_3065105
 Anti-EGFP 1 μg/ml Rabbit polyclonal Sakagami et al. (2005)
 Anti-EGFP 1 μg/ml Guinea pig polyclonal Sakagami et al. (2005)
 Anti-EGFP 1 μg/ml Chicken polyclonal Aves labs GFP-1020 AB_10000240
 Anti-FLAG(M2) 1:3000 Mouse monoclonal Sigma-Aldrich F3165 AB_259529
 Anti-GM130 1 μg/ml 1:1000 Mouse monoclonal BD Transduction Laboratories 610822 AB_398141
 Anti-HA 1 μg/ml Rat monoclonal Sigma-Aldrich 3F10 AB_2314622
 Anti-mCherry 1 μg/ml Rabbit polyclonal Hara et al. (2013)
 Anti-mCherry 1 μg/ml Guinea pig polyclonal Hara et al. (2013) AB_2827679
 Anti-mCherry 1 μg/ml Chicken polyclonal Applied Biologicalalal Materials Y030151
 Anti-N-cadherin 1 μg/ml Guinea pig polyclonal Hara et al. (2016)
 Anti-N-cadherin 1 μg/ml Rabbit polyclonal Takara Bio company M142
 Anti-NeuN 1:1000 Mouse monoclonal CA (pH 6.0), 75°C, 1 h Chemicon MAB377 AB_2298772
 Anti-PSA-NCAM 1:500 Mouse monoclonal Seki and Arai (1991) AB_2315215
 Anti-Rab11 1:250 1:1000 Mouse monoclonal TE (pH 9.0), 75°C, 1 h, BD Transduction Laboratories 610657 AB_397984
 Anti-Sox2 1 μg/ml Rabbit polyclonal CA (pH 6.0), 75°C, 1 h Proteintech
 Anti-STX12 1 μg/ml Rabbit polyclonal Hara et al. (2013)
 Anti-STX12 1 μg/ml 1:1000 Guinea pig polyclonal Hara et al. (2013)
 Anti-STX16 1 μg/ml 0.1 μg/ml Rabbit polyclonal This study
 Anti-TGN38A 1 μg/ml 1:1000 Guinea pig polyclonal Ibuchi et al. (2020)
 Anti-Tuj1 1 μg/ml Guinea pig polyclonal Nittobo Medical Co Ltd. MSFR105990
 Anti-VAMP4 1 μg/ml 1:1000 Mouse monoclonal TE (pH 9.0), 75°C, 1 h, Proteintech 67219-1-Ig AB_2882510
Secondary
 Anti-guinea pig IgG Alexa 488 1:300 Donkey polyclonal Jackson ImmunoResarch 706-545-148 AB_2340472
 Anti-guinea pig IgG Alexa 594 1:300 Donkey polyclonal Jackson ImmunoResarch 706-585-148 AB_2340474
 Anti-guinea pig IgG Alexa 647 1:300 Donkey polyclonal Jackson ImmunoResarch 706-605-148 AB_2340476
 Anti-guinea pig IgG Alexa 594 1:300 Donkey polyclonal Jackson ImmunoResarch 703-545-155 AB_2340375
 Anti-guinea pig IgG Alexa 647 1:300 Donkey polyclonal Jackson ImmunoResarch 703-585-155 AB_2340377
 Anti-guinea pig IgG HRP 1:10,000 Donkey polyclonal Jackson ImmunoResarch 706-036-148 AB_2340448
 Anti-goat IgG Alexa 594 1:300 Donkey polyclonal Jackson ImmunoResarch 705-585-147 AB_2340433
 Anti-mouse IgG Alexa 488 1:300 Donkey polyclonal Thermo Fisher Scientific A-21202 AB_141607
 Anti-mouse IgG Alexa 594 1:300 Donkey polyclonal Thermo Fisher Scientific A-21203 AB_141633
 Anti-mouse IgG Alexa 647 1:300 Donkey polyclonal Jackson ImmunoResarch 715-605-151 AB_2340863
 Anti-mouse IgG HRP 1:10,000 Sheep GE Healthcare NA931 AB_772210
 Anti-rabbit IgG Alexa 488 1:300 Donkey polyclonal Thermo Fisher Scientific A-21206 AB_2535792
 Anti-rabbit IgG Alexa 594 1:300 Donkey polyclonal Thermo Fisher Scientific A-21207 AB_141637
 Anti-rabbit IgG Alexa 647 1:300 Donkey polyclonal Thermo Fisher Scientific A-31573 AB_2536183
 Anti-rabbit IgG HRP 1:10,000 Donkey polyclonal GE Healthcare NA934 AB_772206

CA, citrate buffer; HRP, horseradish peroxidase; TE, Tris-EDTA.