Table 1.
Antibody | Concentration | Species | Antigen retrieval | Source | Catalog number | RRID | |
---|---|---|---|---|---|---|---|
(IHC) | (WB) | ||||||
Primary | |||||||
Anti-Arf4 | 1:200 | 1:1000 | Rabbit polyclonal | Hosoi et al. (2019) | |||
Anti-Arf5 | 1:200 | 1:1000 | Rabbit polyclonal | Hosoi et al. (2019) | |||
Anti-α-Tubulin | 1:3000 | Mouse monoclonal | Sigma-Aldrich | MABT205 | AB_11204167 | ||
Anti-BLBP | 1 μg/ml | Goat polyclonal | Nittobo Medical Co Ltd. | MSFR100290 | AB_2571664 | ||
Anti-BrdU | 1 μg/ml | Mouse monoclonal | 2 N HCl, 37°C, 10 min | BD Transduction Laboratories | 3D4 | AB_2033929 | |
Anti-Cux1 | 1 μg/ml | Rabbit polyclonal | CA (pH 6.0), 75°C, 1 h | Santa Cruz Biotechnology | sc-13024 | AB_2261231 | |
Anti-EEA1 | 1 μg/ml | Rabbit polyclonal | Fukaya et al. (2014) | AB_3065105 | |||
Anti-EGFP | 1 μg/ml | Rabbit polyclonal | Sakagami et al. (2005) | ||||
Anti-EGFP | 1 μg/ml | Guinea pig polyclonal | Sakagami et al. (2005) | ||||
Anti-EGFP | 1 μg/ml | Chicken polyclonal | Aves labs | GFP-1020 | AB_10000240 | ||
Anti-FLAG(M2) | 1:3000 | Mouse monoclonal | Sigma-Aldrich | F3165 | AB_259529 | ||
Anti-GM130 | 1 μg/ml | 1:1000 | Mouse monoclonal | BD Transduction Laboratories | 610822 | AB_398141 | |
Anti-HA | 1 μg/ml | Rat monoclonal | Sigma-Aldrich | 3F10 | AB_2314622 | ||
Anti-mCherry | 1 μg/ml | Rabbit polyclonal | Hara et al. (2013) | ||||
Anti-mCherry | 1 μg/ml | Guinea pig polyclonal | Hara et al. (2013) | AB_2827679 | |||
Anti-mCherry | 1 μg/ml | Chicken polyclonal | Applied Biologicalalal Materials | Y030151 | |||
Anti-N-cadherin | 1 μg/ml | Guinea pig polyclonal | Hara et al. (2016) | ||||
Anti-N-cadherin | 1 μg/ml | Rabbit polyclonal | Takara Bio company | M142 | |||
Anti-NeuN | 1:1000 | Mouse monoclonal | CA (pH 6.0), 75°C, 1 h | Chemicon | MAB377 | AB_2298772 | |
Anti-PSA-NCAM | 1:500 | Mouse monoclonal | Seki and Arai (1991) | AB_2315215 | |||
Anti-Rab11 | 1:250 | 1:1000 | Mouse monoclonal | TE (pH 9.0), 75°C, 1 h, | BD Transduction Laboratories | 610657 | AB_397984 |
Anti-Sox2 | 1 μg/ml | Rabbit polyclonal | CA (pH 6.0), 75°C, 1 h | Proteintech | |||
Anti-STX12 | 1 μg/ml | Rabbit polyclonal | Hara et al. (2013) | ||||
Anti-STX12 | 1 μg/ml | 1:1000 | Guinea pig polyclonal | Hara et al. (2013) | |||
Anti-STX16 | 1 μg/ml | 0.1 μg/ml | Rabbit polyclonal | This study | |||
Anti-TGN38A | 1 μg/ml | 1:1000 | Guinea pig polyclonal | Ibuchi et al. (2020) | |||
Anti-Tuj1 | 1 μg/ml | Guinea pig polyclonal | Nittobo Medical Co Ltd. | MSFR105990 | |||
Anti-VAMP4 | 1 μg/ml | 1:1000 | Mouse monoclonal | TE (pH 9.0), 75°C, 1 h, | Proteintech | 67219-1-Ig | AB_2882510 |
Secondary | |||||||
Anti-guinea pig IgG Alexa 488 | 1:300 | Donkey polyclonal | Jackson ImmunoResarch | 706-545-148 | AB_2340472 | ||
Anti-guinea pig IgG Alexa 594 | 1:300 | Donkey polyclonal | Jackson ImmunoResarch | 706-585-148 | AB_2340474 | ||
Anti-guinea pig IgG Alexa 647 | 1:300 | Donkey polyclonal | Jackson ImmunoResarch | 706-605-148 | AB_2340476 | ||
Anti-guinea pig IgG Alexa 594 | 1:300 | Donkey polyclonal | Jackson ImmunoResarch | 703-545-155 | AB_2340375 | ||
Anti-guinea pig IgG Alexa 647 | 1:300 | Donkey polyclonal | Jackson ImmunoResarch | 703-585-155 | AB_2340377 | ||
Anti-guinea pig IgG HRP | 1:10,000 | Donkey polyclonal | Jackson ImmunoResarch | 706-036-148 | AB_2340448 | ||
Anti-goat IgG Alexa 594 | 1:300 | Donkey polyclonal | Jackson ImmunoResarch | 705-585-147 | AB_2340433 | ||
Anti-mouse IgG Alexa 488 | 1:300 | Donkey polyclonal | Thermo Fisher Scientific | A-21202 | AB_141607 | ||
Anti-mouse IgG Alexa 594 | 1:300 | Donkey polyclonal | Thermo Fisher Scientific | A-21203 | AB_141633 | ||
Anti-mouse IgG Alexa 647 | 1:300 | Donkey polyclonal | Jackson ImmunoResarch | 715-605-151 | AB_2340863 | ||
Anti-mouse IgG HRP | 1:10,000 | Sheep | GE Healthcare | NA931 | AB_772210 | ||
Anti-rabbit IgG Alexa 488 | 1:300 | Donkey polyclonal | Thermo Fisher Scientific | A-21206 | AB_2535792 | ||
Anti-rabbit IgG Alexa 594 | 1:300 | Donkey polyclonal | Thermo Fisher Scientific | A-21207 | AB_141637 | ||
Anti-rabbit IgG Alexa 647 | 1:300 | Donkey polyclonal | Thermo Fisher Scientific | A-31573 | AB_2536183 | ||
Anti-rabbit IgG HRP | 1:10,000 | Donkey polyclonal | GE Healthcare | NA934 | AB_772206 |
CA, citrate buffer; HRP, horseradish peroxidase; TE, Tris-EDTA.