FIG. 1.

Influence of culture temperature on expression of genes for AF and ST biosynthesis. Supplemented minimal medium was used with 3.7 g of NH₄Cl per liter for A. parasiticus and 6 g of NaNO₃ per liter for A. nidulans. Each of the above probes was from the relevant cloned gene in A. nidulans and A. parasiticus. The A. parasiticus probes were a 4.5-kb KpnI fragment for pksA and a cloned β-tub gene (6a) as an internal control. The A. nidulans probes were a 5.4-kb KpnI fragment for stcA (3, 13) and a 5.6-kb KpnI fragment for aflR, stcE, and stcD (3).