Table 1.
Primers employed for reverse transcription-quantitative polymerase chain reaction experiments to determine the expression of β-catenin, c-Myc and TFG-β, and the genes used as reference genes
Name
|
Primer
|
Sequence
|
Product Lenght
|
Ref.
|
TGF-β | Forward | 5’- TACCTGAACCCGTGTTGCTCTC-3’ | 122 | [10] |
Reverse | 5’- GTTGCTGAGGTATCGCCAGGA-3’ | |||
β-catenin | Forward | 5’- CACAAGCAGAGTGCTGAAGGTG-3’ | 146 | [11] |
Reverse | 5’- GATTCCTGAGAGTCCAAAGACAG-3’ | |||
c-MYC | Forward | 5’-GCCACGTCTCCACACATCAG-3’ | 132 | [12] |
Reverse | 5’-TGGTGCATTTTCGGTTGTTG-3’ | |||
B2M | Forward | 5’-GTGCTCGCGCTACTCTCTC-3’ | 150 | [13] |
Reverse | 5’-GTCAACTTCAATGTCGGAT-3’ | |||
PPIA | Forward | 5’-GCAAATGCTGGACCCAACACAAAT-3’ | 174 | [14] |
Reverse | 5’-AATGGTGATCTTCTTGCTGGTCTTG-3’ | |||
RPLP0 | Forward | 5’-GCAATGTTGCCAGTGTCTG-3’ | 142 | [15] |
Reverse | 5’-GCCTTGACCTTTTCAGCAA-3’ |