| Cycle number | Denature | Anneal | Extend |
|---|---|---|---|
| 1 | 98°C, 30 s | ||
| 2-[cycle number determined in Step 29] | 98°C, 10 s | 72°C, 30 s | 72°C, 30 s |
| [cycle number determined in Step 29] + 1 | 72°C, 2 min |
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