FIGURE 1.

Malignant cervical epithelial cells exhibit no remarkable signs of tumor escape. (A) UMAP projection of 119,351 cells from 17 clinical samples consisting of normal cervical tissues, HSIL foci, and CC tissues that were clustered into 32 clusters. Each dot corresponds to a single cell and is colored according to the cell cluster. (B) UMAP projection of 119,351 cells, which were further categorized into 11 major cell types. Each dot, which corresponds to a single cell, is colored according to cell type. See also Supplementary Figure S1A. (C) UMAP projection of the 24 epithelial subclusters generated from unsupervised reclustering. Each dot, which corresponds to a single epithelial cell, is colored according to subcluster. See also Supplementary Figure S1B. (D) CNV profiles of epithelial cells inferred from scRNA‐seq of normal cervical tissues (N1 with HPV infection [Normal 1_HPV+]), HSIL_2, and SCC_4 data. Red and blue indicate chromosomal amplifications and deletions, respectively. (E) UMAP projection of all epithelial cells presenting as malignant (light blue) and non‐malignant (red). (F) UMAP projection of all epithelial cells presenting at different disease stages, including those in the normal cervix (N), HSIL, and CA (cancer). Each dot, which corresponds to a single cell, is colored according to disease stage. (G‐J) Dot plot indicating the average expression levels and proportions of cells expressing tumor‐associated antigen‐related adhesion molecules (G), antigen processing‐related genes (H), MHC‐I molecules (I), and ligands for immune checkpoints (J) in malignant and non‐malignant epithelial cells. The colors represent the average expression levels, and dot sizes represent the percent expression of selected genes. Abbreviations: UMAP, Uniform Manifold Approximation and Projection; HSIL, high‐grade squamous intraepithelial lesion; CNV, copy number variation; scRNA‐seq, single‐cell RNA sequencing; HPV, human papillomavirus; SCC, squamous cell carcinoma; MHC, major histocompatibility complex.