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. 2023 Jul 14;278(6):e1313–e1326. doi: 10.1097/SLA.0000000000006005

FIGURE 3.

FIGURE 3

MITO improves mitochondrial metabolism after IRI. Normal healthy human conditionally immortalized proximal tubular cells (ciPTECs) (NT) or IRI damaged ciPTECs were treated without (IRI) or with mitochondrial transplantation (MITO) (20 µg/ml, IRI+MITO). A, MITO normalized reactive oxygen species (ROS) levels, mitochondrial thiobarbituric reactive substances and JC-1 membrane potential altered by IRI. B, Key tricarboxylic acid (TCA) cycle enzyme [succinate dehydrogenase (DH), citrate synthase, malate DH] activities in IRI ciPTECs were significantly increased by MITO (IRI+MITO). C, Electron transport chain (ETC) and adenosine 5′-triphosphate (ATP) concentrations in IRI ciPTECs were significantly increased with MITO treatment (IRI+MITO). Data are mean ±SD of 4 different experiments, one-way analysis of variance (ANOVA) with Tukey multicomparison test was performed: *P< 0.05, **P < 0.01, ***P < 0.001.