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C. cayetanensis and Eimeria amplified products from nested PCR (lanes 2 to 5) and RFLP fragments from MnlI digestion of those PCR amplified products (lanes 7 to 10). Lanes 1, 6, and 11, molecular size standards; lanes 2 and 7, negative control; lanes 3 and 8, Universidad Peruana Cayetano Heredia C. cayetanensis positive control; lanes 4 and 9, C. cayetanensis from wastewater; lanes 5 and 10, Eimeria spp. (U of A) positive control. Primers migrated to point P.
