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. 2023 Oct 6;34(11):1889–1899. doi: 10.1681/ASN.0000000000000219

Figure 3.

Figure 3

Effects of APOL1 p.N264K on protein function. (A) APOL1 disease variants, G1 and G2, are toxic when overexpressed in human immortalized podocytes. APOL1 G0 overexpression does not affect podocyte cell viability. Error bars represent SEM. (B) The cytotoxicity of APOL1 G1 and G2 risk variants is attenuated by the N264K mutation. Error bars represent SEM. (C) G2 APOL1-mediated calcium transit is blocked by the N264K mutation in HEK cells. The expression of APOL1 G2 or APOL1 G2 with p.N264K was induced with doxycycline, and fluorescence was measured in the presence of increasing concentrations of calcium. The fluorescence level in (C) is a measure of intracellular calcium. Error bars represent SD. HEK, human embryonic kidney.