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. 2023 Nov 8;18(11):e0291302. doi: 10.1371/journal.pone.0291302

Fig 3. Maximum likelihood tree generated by MEGA X software based on Kimura-2 parameter model was used for the multiple alignments of partial msp4 sequences (313 bp) from Anaplasma ovis isolated in this study and those available in GenBank from other countries across the world.

Fig 3

Anaplasma marginale (KU497715), Anaplasma centrale (AF428090) and Anaplasma phagocytophilum (EU008082) were used as an out group. The three new sequences of A. marginale obtained are highlighted in red boxes. Scale bar represents 0.10 substitutions per nucleotide position. Number on the node represents bootstrap value.