Fig. 4. Developmental principles across distinct human brain regions.

(A) scRNAseq data from hypothalamus, cortex, and ganglionic eminence for three matched human embryonic samples at GW18, GW19, and GW20. Cell types presented in 3D UMAP (https://tinyurl.com/2p9yzw5h), inset panels are rotations of UMAP showing additional clusters. (B) 3D UMAP (https://tinyurl.com/3yazd898) showing overlay of samples by brain region. (C) Distribution of cells within cell-type cluster by brain region. (D) Polar plot of TF expression across three brain regions for dividing progenitor cells. Points off-axis reflect shared expression between regions. Gray points indicate no significant difference between regions. (E) Heatmap depicting genes with similar or divergent expression patterns across neuronal lineages. Colors represent scaled expression of cells only within a given cell-type and brain region, as described in x-axis legend. (F) Subsets of GRNs for hypothalamus, cortex, and ganglionic eminence were created on the basis of genes expressed in neural progenitor cells, radial glia, and dividing progenitors. Resulting overlapping networks show edges from hypothalamus network (blue) are roughly evenly split and adjacent to the ganglionic eminence network (green) and cortex network (red). Shared edges follow the color scheme introduced in (D). Top 20 TFs with the greatest number of connections are highlighted in white.