Table II.
Gene | Primer sequence |
---|---|
GAPDH | F: 5′-GGGAGCCAAAAGGGTCAT-3′ |
R: 5′-GAGTCCTTCCACGATACCAA-3′ | |
DLX5 | F: 5′-ATGACAGGAGTGTTTGACAGAAG-3′ |
R: 5′-CTAATAGAGTGTCCCGGAGGCCA-3′ | |
DLX5 ChIP primer 1 | F: 5′-TTCTACACTCGCCTTTGGTG-3′ |
R: 5′-CAGCACAAGGCTCTGTGATG-3′ | |
DLX5 ChIP primer 2 | F: 5′-CCCACTCCACAACAAGCAA-3′ |
R: 5′-GCACAGCCTTGGTTAAATCC-3′ |
RT-qPCR, reverse transcription-quantitative polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; DLX5, distal-less homeobox gene 5; ChIP, chromatin immunoprecipitation; F, forward; R, reverse.