TABLE 1.
Range of organophosphonate substrates utilized by B. cepacia Pal6 as the sole phosphorus source
| Substrate (1 mM) | Growth (μg of protein/ml)a |
|---|---|
| Inorganic phosphate | 200 |
| 2-Phosphonopropionic acid | 200 |
| 2AEPb | 200 |
| Phenylphosphonic acid | 160 |
| Hydroxymethylphosphonic acid | 160 |
| Methylphosphonic acid | 120 |
| Phosphonoacetic acid | 120 |
| 1-Aminobutylphosphonic acid | 30 |
| Aminomethylphosphonic acid | 30 |
| 3-Aminopropylphosphonic acid | 20 |
| Ethylphosphonic acid | 10 |
| 2-Amino-4-phosphonobutyric acid | 10 |
| Phosphonoformic acid | 10 |
| 4-Aminobutylphosphonic acid | 10 |
| 1-Aminoethylphosphonic acid | 10 |
| Phosphate-free medium | 0 |
Results were scored negative if the protein yield, as measured by the method of Binks et al. (1), was less than 20% of that of the positive control containing 1 mM inorganic phosphate. Results are means of duplicates which on no occasion varied by more than 5%.
2AEP was also metabolized as the sole carbon, nitrogen, and phosphorus source.