TABLE 1.
Selected acetate kinetic parameters of D. acetoxidans, D. amnigenus, and the two genera of acetate-degrading methanogens
| Organism | Substrate utilization | μmax (day−1) | Vmax (μmol min−1 g of protein−1) | Km (mM) | Threshold concn (μM) | Reference and/ or source |
|---|---|---|---|---|---|---|
| D. acetoxidans | Specialist | 0.31–0.41 | 43 ± 14a | 0.6 ± 0.4a | <15 | 9; this study |
| D. amnigenus | Generalist | 0.14–0.20 | 28 ± 7a | 0.6 ± 0.4a | <15 | 8; this study |
| Methanosarcina sp. | Generalist | 0.46–0.69 | —b | 3.0 | 190–1,180 | 4, 7 |
| Methanosaeta | ||||||
| M. soehngenii | Specialist | 0.08–0.29 | 76 | 0.4–0.7 | 7–69 | 4, 6, 7 |
| M. concilii | Specialist | 0.21–0.69 | 32 | 0.8–1.2 | — | 6, 7 |
| Strain MTAS | Specialist | 0.37 | 170 | 0.5 | — | 5, 6 |
| Strain MTKO | Specialist | 0.38 | 98 | 1.17 | — | 5, 6 |
Mean of four independent experiments ± standard deviation.
—, not determined.