Table III.
In vivo clinical outcomes and limb perfusion of ischemic hindlimb mouse model compared between the quality and quantity media-cultured mononuclear cell (QQMNC) and peripheral blood mononuclear cell (PBMNC) groups
| Evaluated parameters | PBMNC group | QQMNC group | P value |
|---|---|---|---|
| Overall (n = 40) | (n = 20) | (n = 20) | |
| Limb appearance | .096 | ||
| Normal appearing limb | 10 (50) | 16 (80) | |
| Limb cyanosis | 6 (30) | 4 (20) | |
| Limb gangrene | 4 (20) | 0 (0) | |
| Complication | 0 (0) | 0 (0) | |
| Laser Doppler perfusion imaging (aPU) | |||
| After ligation | 334.8 ± 126.0 | 283.0 ± 119.0 | .301 |
| 14-days post-MNC transplantation | 434.3 ± 230.7 | 641.9 ± 232.2 | .020 |
| High arterial ligation model (n = 16) | (n = 8) | (n = 8) | |
| Limb appearance | .041 | ||
| Normal appearing limb | 1 (12.5) | 6 (75.0) | |
| Limb cyanosis | 4 (50.0) | 2 (25.0) | |
| Limb gangrene | 3 (37.5) | 0 (0.0) | |
| Laser Doppler perfusion imaging (aPU) | |||
| After ligation | 213.4 ± 132.4 | 168.1 ± 103.4 | .574 |
| 14-days post-MNC transplantation | 258.3 ± 154.5 | 468.9 ± 225.3 | .038 |
| Low arterial ligation model (n = 24) | (n = 12) | (n = 12) | |
| Limb appearance | .96 | ||
| Normal appearing limb | 9 (75.0) | 10 (83.3) | |
| Limb cyanosis | 2 (16.7) | 2 (16.7) | |
| Limb gangrene | 1 (8.3) | 0 (0.0) | |
| Laser Doppler perfusion imaging (aPU) | |||
| After ligation | 386.2 ± 90.5 | 343.8 ± 88.6 | .291 |
| 14-days post-MNC transplantation | 501.2 ± 219.7 | 702.0 ± 242.4 | .114 |
aPU, Arbitrary perfusion unit; MNC, mononuclear cell.
Values are number (%) or mean ± standard deviation.
Boldface entries indicate statistical significance.