Fig. 2. Commercial resins do not impact cell viability compared to standard photolithography-based microwells and drug response in 3D printed microwells reflects traditional microplate results.

a Annotated CAD model of microwell array dimensions. b Representative images of PDMS from 3D-printed molds for tested resins. c Viability after 4 days of cancer cells seeded in PDMS from 3D-printed molds. d Representative images of tumor cells (red) seeded in microwells after 4 days. Green represents dead cells. e Viability after 4 days of cancer cells seeded in PDMS of treatment with 100 nM paclitaxel. Statistical analysis performed using Student’s t test. Data are representative of at least two independent biological replicates. f Representative images of tumor cells seeded in microwells after paclitaxel treatment Green represents dead cells. Scale bars represent 25 μm. Error bars denote SEM