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. 2023 Oct 25;623(7986):356–365. doi: 10.1038/s41586-023-06671-8

Fig. 4. Reward DAN activity reduces subsequent need seeking.

Fig. 4

a, Left, schematics and experimental protocol. Right, starved flies trained with 0273-neuron activation (orange) disregard sucrose to seek the CS+ odour predicting artificial reward, whereas flies trained with sucrose exhibit no preference, and mock-trained flies prefer sucrose (left to right: n = 11, 10 and 10). b, Starved flies trained with activation of β′2&γ4 DANs also disregard sucrose to seek artificial reward (n = 10, unconditioned stimulus is the red light protocol from a and genotype corresponds to the schematic with blue regions in a). Different letters above bars in a,b indicate groups that are significantly different from each other (P < 0.05; one-way ANOVA then Tukey’s HSD). c, Left, schematics and protocol. Right, activation of 0273 neurons, R48B04 DANs, or β′2&γ4 DANs in an odourless tube decreases subsequent sucrose approach in starved flies (left to right: n = 26, 26, 26, 26, 28, 28, 24, 24, 20 and 20). Two-way ANOVA then multiple comparisons with Šidák’s correction. Data are mean ± s.e.m.; dots are individual data points that correspond to independent behavioural experiments. Exact statistical values and comparisons are presented in Supplementary Information.