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. 2023 Nov 5;13(21):e4861. doi: 10.21769/BioProtoc.4861

Figure 1. Biosynthesis of serine in E. coli.

Figure 1.

In this protocol, a ∆serC mutant expression host is used as it lacks the ability to biosynthesize pSer amino acid, which would compete with nhpSer encoding. SerB is also overexpressed to hydrolyze any free pSer amino acid that might enter the cell from the media or be formed by promiscuous transaminases that can substitute for SerC function. For GCE systems that encode authentic phosphoserine, ∆serB expression hosts are used in order to build up intracellular pSer concentrations.