| Reagent | Final concentration (of 1×) | Quantity |
| Tryptone | 1.2% (w/v) | 12 g |
| Yeast extract | 2.4% (w/v) | 24 g |
| Glycerol (50% [v/v]) | 0.5% (v/v) | 10 mL |
| H2O | n/a | To 900 mL |
| Total | n/a | 900 mL |
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