Fig. 3.

LASSO predictive model identifies a 13 pEV miRNA MTC diagnostic signature. a Parameter selection in LASSO regression and 13 pEV miRNA signature. b ROC curve of 13 pEV miRNAs (AUC = 1.00 p < 0.0001) and application of expression and significance criteria identifying pEV miR-26b-5p and miR-451a as putative biomarkers. c ROC curve of 2 pEV miRNAs, miR-26b-5p and miR-451a, (AUC = 0.8775, p < 0.0001). Blue line = sensitivity, red line = identity. d Representative image of in situ hybridization (RNAscope^®) showing the expression of miR-451a and miR-26b in MTC tissue samples (dot-like pattern staining). Magnification 10X, scale bar 100 μm; inset at 20X, scale bar 50 μm