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. 2023 Nov 8;13:19383. doi: 10.1038/s41598-023-46614-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Glucose restriction does not change the conformation of the 20S proteasome. (A) Representative cryo-transmission electron microcopy (cryo-TEM) micrographs of 20S proteasome particles isolated from cells cultured under control (PT-C) or glucose restriction (PT-GR) conditions. (B) 2D classification of pore opening and frequency distribution in experimental samples performed with cryoSPARC software. (C–F) PT-C or PT-GR samples were analyzed by SAXS as described in experimental procedures. (C) Scattering curves, (D) distance pair distribution function, p(r), (E) fitting of scattering curves using a form factor of hollow cylinder, and (F) hollow cylinder modelling results. (G) Circular dichroism curves obtained for PT-C or PT-GR samples as described in experimental procedures. The data is representative of 2 independent experiments.