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. 2023 Nov 8;13:19383. doi: 10.1038/s41598-023-46614-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Glucose restriction induces the phosphorylation of at least one residue in α5 proteasome subunit. MS (A) and MS/MS (C) spectra of the peptide ATSPLLESDSIEK without phosphorilation on site 2 or 3. MS (B) and MS/MS (D) spectra of the peptide ATSPLLESDSIEK with phosphorilation on site 2 or 3. (E) Top view representation of the three-dimensional structure of the 20S proteasome (pdb entry 3D29). The position of the Thr55 residue is highlighted in red. The image was created on the protein data bank website, combining cartoon and molecular surface visualization⁴¹.