Table 2.

Buffers used in proteasome purification and enzymatic assays.

Buffer number	Composition (mM)
	Tris–Cl pH 7.5	Hepes pH 7.2	MgCl2	NaCl	KCl	Imidazole
1	50	–	5	500	20	20
2	50	–	5	500	20	400
3	–	10	5	–	100	–
4	–	10	5	–	1000	–
5	50	–	5	–	–	–