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. 2023 Nov 8;14:7194. doi: 10.1038/s41467-023-42963-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, b β-catenin activates NFκB via Tlr4. a Embryos were injected with control (mKO2) or constitutively active β-catenin mutant (β-cat CA) mRNA, with or without Tlr4 DN mRNA. b Embryos were injected with control or ctnnb2 (zebrafish β-catenin) MO. WISH for dGFP in NFκB-tkP:dGFP-transgenic; frzb in WT embryos at the dome stage, animal view. Scale bar = 200 μm. c NFκB is activated following Wnt/β-catenin activation in the developing dorsal organizer. WISH for dGFP in OTM:d2EGFP and NFκB-tkP:dGFP-transgenic embryos and frzb in WT embryos at the indicated stage. The dGFP-expressing and frzb-expressing dorsal regions are indicated by red arrowheads. Scale bar = 200 μm. d β-catenin activates wnt5b expression in early embryos. Embryos were injected with control (mKO2) or constitutively active β-catenin mutant (β-catCA) mRNA. WISH for wnt5a and wnt5b at the sphere stage, animal view. Scale bar = 200 μm. e, f Wnt/β-catenin signaling activates NFκB via Wnt5b. e Embryos were injected with ctrl or wnt5b MO. f Embryos were injected with control (mKO2) or constitutively active β-catenin mutant (β-cat CA) mRNA, with or without wnt5b MO. WISH for dGFP in NFκB-tkP:dGFP-transgenic embryos at the dome stage, animal view. Scale bar = 200 μm. g, h Wnt5b activates frzb expression through Tlr4/Rel. g Embryos were injected with control (mKO2) or wnt5b mRNA, and co-injected with Tlr4 DN mRNA or rel MO. WISH for dGFP in NFκB-tkP:dGFP-transgenic, frzb in WT embryos at the dome stage, animal view. Scale bar = 200 μm. h WISH for dharma in WT embryos injected with control (mKO2) or wnt5b mRNA at 30% epiboly stage. Scale bar = 200 μm. Box plots of the angle of marker gene show first and third quartile, median is represented by a line, whiskers indicate the minimum and maximum. Each dot represents one embryo. P-values for unpaired two-tailed t-tests are indicated. Source data are provided as a Source Data file.