Table 2.
Pan-rodent Ppia and viral target multiplex assay primer sequences.
| Multiplex assay primer sequences | ||
|---|---|---|
| Oligo name | Primer sequence (5′-3′) | Conc. (µM) |
| Pan_Ppia_Fwd | CCCACCGTGTTCTTCGAC | 400 |
| Pan_Ppia_Rev | TCCTTTCTCTCCAGTGCTCAG | 400 |
| Pan_Ppia_Prb | GAGCCCTTGGGCCGCGTCTC | 200 |
| CCHFV_Fwd | CAGGACATGGACATAGTGGC | 400 |
| CCHFV_Rev | ATTGCCCTTGACGTTGTAGG | 400 |
| CCHFV_Prb | CCCTTGTTGGCAAGCAATCCC | 200 |
| NiV_Fwd | CTGGTCTCTGCAGTTATCACCATCGA | 400 |
| NiV_Rev | ACGTACTTAGCCCATCTTCTAGTTTCA | 400 |
| NiV_Prb | CAGCTCCCGACACTGCCGAGGAT | 200 |
| LASV_Fwd | GGAAGCCACAGAAAGCTGAC | 400 |
| LASV_Rev | GGAGTGCATCAATGACAGCA | 400 |
| LASV_Prb | AAATCCCTGCAGTCGGCAGG | 200 |
GenBank accession numbers used to produce a consensus sequence for designing primers and probes against Ppia from multiple rodent species were NM_008907 (mouse), XM_005086775 (hamster), and XM_003465805 (guinea pig). Primer sequences are indicated in the 5′–3′ direction. The final concentration (conc.) of each individual primer or probe per reaction is indicated in micromolar (μM).