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. 2023 Nov 8;8:64. doi: 10.1038/s41536-023-00339-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a B6-Ahr^fl/fl mice and B6-Ahr^fl/fl;Foxn1-cre mice, pretreated by 5.5 Gy TBI, were intraperitoneally injected with vehicle or rmIL-22 (200 ng) thrice weekly for 2 weeks. At day 14, thymus cells were counted as described (n = 5). b, c Thymii were collected from irradiated mice. qPCR was performed to detect mRNA levels of IL-22 and IL-22RA1 (n = 4). The values of control groups were set as 1, and the values of other groups were 2^–ΔΔCT relative to controls. d Thymic stromal cells were isolated from irradiated mice (n = 3). Western blot was performed with indicated antibodies. Band intensity was analyzed using ImageJ. e Cryosections of thymii were stained by immunofluorescence with anti-IL-22RA1 (n = 4). Fluorescence intensity was analyzed using ImageJ. Scale bar: 50 μm. Data are mean ± SD, compared using one-way ANOVA test or Student’s t test. *, p < 0.05; **, p < 0.01; ***, p < 0.001; n.s., not significant.