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. 2023 Nov 8;8:64. doi: 10.1038/s41536-023-00339-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, b Thymic stromal cells were isolated from wild-type C57BL/6 mice treated by 5.5 Gy TBI. Day 0 indicates the samples were collected right before TBI. a qPCR was performed to detect mRNA levels (n = 5). The values of Day 0 were set as 1. b Western blot was performed with indicated antibodies. c, d B6-Il22ra1^fl/fl mice and B6-Il22ra1^fl/fl;Foxn1-cre mice, pretreated by 5.5 Gy TBI, were intraperitoneally injected with vehicle, rmIL-22 (200 ng) or FICZ (0.1 mg/kg) thrice weekly for 2 weeks. At day 14, c thymus cells were counted as described (n = 5). d H&E staining was performed on slides of thymus. Scale bar: 500 μm. Data are mean ± SD, compared using one-way ANOVA test or Student’s t test. *, p < 0.05; **, p < 0.01; ***, p < 0.001; n.s., not significant.