Table 1. Genome data for Bombus pratorum, iyBomPrat1.1.
| Project accession data | ||
|---|---|---|
| Assembly identifier | iyBomPrat1.1 | |
| Species | Bombus pratorum | |
| Specimen | iyBomPrat1 | |
| NCBI taxonomy ID | 30194 | |
| BioProject | PRJEB48116 | |
| BioSample ID | SAMEA7520485 | |
| Isolate information | iyBomPrat1, female, head and thorax (genome sequencing); abdomen (Hi-C)
iyBomPrat2, female (RNA sequencing) |
|
| Assembly metrics * | Benchmark | |
| Consensus quality (QV) | 57.3 | ≥ 50 |
| k-mer completeness | 99.99% | ≥ 95% |
| BUSCO ** | C:97.7%[S:97.4%,D:0.3%],
F:0.4%,M:1.9%,n:5,991 |
C ≥ 95% |
| Percentage of assembly mapped to chromosomes | 96.23% | ≥ 95% |
| Sex chromosomes | Not applicable | localised homologous pairs |
| Organelles | Mitochondrial genome assembled | complete single alleles |
| Raw data accessions | ||
| PacificBiosciences SEQUEL II | ERR7123979 | |
| 10X Genomics Illumina | ERR7113571–ERR7113574 | |
| Hi-C Illumina | ERR7113575 | |
| PolyA RNA-Seq Illumina | ERR7113576, ERR7113577 | |
| Genome assembly | ||
| Assembly accession | GCA_930367275.1 | |
| Accession of alternate haplotype | GCA_930367225.1 | |
| Span (Mb) | 285.1 | |
| Number of contigs | 90 | |
| Contig N50 length (Mb) | 8.5 | |
| Number of scaffolds | 50 | |
| Scaffold N50 length (Mb) | 16.5 | |
| Longest scaffold (Mb) | 22.7 | |
| Genome annotation | ||
| Number of protein-coding genes | 13,746 | |
| Number of non-coding genes | 5,678 | |
| Number of gene transcripts | 40,821 | |
* Assembly metric benchmarks are adapted from column VGP-2020 of “Table 1: Proposed standards and metrics for defining genome assembly quality” from ( Rhie et al., 2021).
** BUSCO scores based on the hymenoptera_odb10 BUSCO set using v5.3.2. C = complete [S = single copy, D = duplicated], F = fragmented, M = missing, n = number of orthologues in comparison. A full set of BUSCO scores is available at https://blobtoolkit.genomehubs.org/view/iyBomPrat1.1/dataset/CAKNEW01/busco.