|
Experimental models
|
| C57BL/6J (M. musculus) |
Envigo |
C57BL/6NHsd |
|
Recombinant DNA
|
|
pD2109‐CMV‐GFP
|
ATUM |
361593 |
|
Antibodies
|
| CD34:FITC |
BD Biosciences |
#562608 |
| VEGFR2:PE |
BD Biosciences |
#121905 |
| VEGFR1:Alexa594 |
Novus Biological |
#NB100‐527AF594 |
| CD11b: BUV395 |
BD Biosciences |
#565976 |
| ACSA2:APC |
Miltenyi Biotec |
#130‐117‐535 |
|
Oligonucleotides and sequence‐based reagents
|
|
Gapdh (F)
|
This study |
5′‐CATCACTGCCACCCAGAAGACTG‐3′ |
|
Gapdh (R)
|
This study |
5′‐ATGCCAGTGAGCTTCCCGTTCAG‐3′ |
|
Fabp7 (F)
|
This study |
5′‐TGGGAAACGTGACCAAACCA‐3′ |
|
Fabp7 (R)
|
This study |
5′‐AGCTTGTCTCCATCCAACCG‐3′ |
|
Trf (F)
|
This study |
5′‐AGACTTCGAGTTGCTCTGCC‐3′ |
|
Trf (R)
|
This study |
5′‐CAGAAATTGCCGGTGCAGTC‐3′ |
|
Dbi (F)
|
This study |
5′‐TGCGCTCTGTGACTTGATTG‐3′ |
|
Dbi (R)
|
This study |
5′‐ATCGCCCACAGTAGCTTGTTT‐3′ |
|
Software
|
| Spectraflo 1.0.5 |
https://cytekbio.com/pages/spectro‐flo
|
|
| CellNeighborEX |
https://github.com/hkim240/CellNeighborEX
|
|
| Seurat 3.2.2 |
https://satijalab.org/seurat/
|
|
| Scanpy 1.9.1 |
https://github.com/scverse/scanpy
|
|
| Squidpy 1.2.3 |
https://github.com/scverse/squidpy
|
|
| RCTD (Spacexr) |
https://github.com/dmcable/spacexr
|
|
| NicheNet 1.0.0 |
https://github.com/saeyslab/nichenetr
|
|
| DoubletFinder 2.0 |
https://github.com/chris‐mcginnis‐ucsf/DoubletFinder
|
|
| clusterProfiler 4.6.0 |
https://github.com/YuLab‐SMU/clusterProfiler
|
|
|
Other
|
| Cytek Aurora Cell Sorter |
Cytekbio |
|
| QuantStudioTM 5 |
Applied Biosystems |
|
| PureLink RNA Mini kit |
Thermo Fisher Scientific |
#12183020 |
| PowerUp SYBR Green master mix |
Thermo Fisher Scientific |
#A25741 |
| Superscript Vilo cDNA synthesis kit |
Thermo Fisher Scientific |
#11755050 |