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. 2023 Oct 10;19(11):e11670. doi: 10.15252/msb.202311670
Reagent/Resource Reference or source Identifier or catalog number
Experimental models
C57BL/6J (M. musculus) Envigo C57BL/6NHsd
Recombinant DNA
pD2109‐CMV‐GFP ATUM 361593
Antibodies
CD34:FITC BD Biosciences #562608
VEGFR2:PE BD Biosciences #121905
VEGFR1:Alexa594 Novus Biological #NB100‐527AF594
CD11b: BUV395 BD Biosciences #565976
ACSA2:APC Miltenyi Biotec #130‐117‐535
Oligonucleotides and sequence‐based reagents
Gapdh (F) This study 5′‐CATCACTGCCACCCAGAAGACTG‐3′
Gapdh (R) This study 5′‐ATGCCAGTGAGCTTCCCGTTCAG‐3′
Fabp7 (F) This study 5′‐TGGGAAACGTGACCAAACCA‐3′
Fabp7 (R) This study 5′‐AGCTTGTCTCCATCCAACCG‐3′
Trf (F) This study 5′‐AGACTTCGAGTTGCTCTGCC‐3′
Trf (R) This study 5′‐CAGAAATTGCCGGTGCAGTC‐3′
Dbi (F) This study 5′‐TGCGCTCTGTGACTTGATTG‐3′
Dbi (R) This study 5′‐ATCGCCCACAGTAGCTTGTTT‐3′
Software
Spectraflo 1.0.5 https://cytekbio.com/pages/spectro‐flo
CellNeighborEX https://github.com/hkim240/CellNeighborEX
Seurat 3.2.2 https://satijalab.org/seurat/
Scanpy 1.9.1 https://github.com/scverse/scanpy
Squidpy 1.2.3 https://github.com/scverse/squidpy
RCTD (Spacexr) https://github.com/dmcable/spacexr
NicheNet 1.0.0 https://github.com/saeyslab/nichenetr
DoubletFinder 2.0 https://github.com/chris‐mcginnis‐ucsf/DoubletFinder
clusterProfiler 4.6.0 https://github.com/YuLab‐SMU/clusterProfiler
Other
Cytek Aurora Cell Sorter Cytekbio
QuantStudioTM 5 Applied Biosystems
PureLink RNA Mini kit Thermo Fisher Scientific #12183020
PowerUp SYBR Green master mix Thermo Fisher Scientific #A25741
Superscript Vilo cDNA synthesis kit Thermo Fisher Scientific #11755050