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. 2023 Oct 26;186(22):4803–4817.e13. doi: 10.1016/j.cell.2023.08.017

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© 2023 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Figure S1 — Development and optimization of the Se transformation protocol, related to Figure 2

(A) Growth of Ai (left) and Se (right) in co-cultures containing the indicated concentrations of hygromycin. Data represent mean ± SD.

(B and C) Quantification of Se, Ai, and transformed Se populations over the course of our transformation protocol (see Figure 2B) with varying lengths (B) or concentrations (C) of transforming DNA. Transformations were performed in the presence (top panels) and absence (bottom panels) of selection for transformants with hygromycin.