Fig. 3.

Srg3 knockdown inhibited LPS-induced BEAS-2B cell death. A, B qRT–PCR and western blot analysis were performed to detect the mRNA and protein expression levels of Srg3 in BEAS-2B cells. C, D CCK-8 and EdU staining were used to analyze the growth activity of BEAS-2B cells. E DAPI staining was used to analyze the nuclear morphology of BEAS-2B cells. F BEAS-2B and PMA-treated THP-1 cells were cocultured in a Transwell system, and THP-1 cells were collected for subsequent experiments. G Flow cytometry was used to detect the proportion of CD86 and CD206 in THP-1 cells after coculture. H Immunofluorescence staining was used to detect the fluorescence intensity of iNOS and Arg1 in THP-1 cells after coculture. Each experiment was repeated three times, and the data were presented in the form of mean plus or minus standard deviation. One-way ANOVA or two-way ANOVA was used for significance analysis between the data. After ANOVA, Tukey’s multiple comparison test was used for post hoc test. **P < 0.01, ***P < 0.001, ***P < 0.0001