Table 1.
Details of primer sequences, amplicon length, annealing temperatures and references of the primers used to amplify the cytochrome oxidase 1 gene (cox1) in this study
| Primer pair | References | Function | Sequence (5´–3´) | Tm (°C) | Amplicon lenght (bp) |
|---|---|---|---|---|---|
| C1-J-1718 | Simon et al. [12] | Forward | GGAGGATTTGGAAATTGATTAGTTCC | 50 | 850 |
| H7005P-R | Percy and Cronk [36] | Reverse | TGAGCTACTACRTARTATGTRTCATG | ||
| LCOP-F | From this study | Forward | AGAACWAAYCATAAAAYWATTGG | 48 | 658 |
| HCO2198 | Folmer et al. [11] | Reverse | TAAACTTCAGGGTGACCAAAAAATCA | ||
| LepF1 | Hebert et al. [13] | Forward | ATTCAACCAATCATAAAGATATTGG | 50 | 658 |
| LepR1 | Hebert et al. [13] | Reverse | TAAACTTCTGGATGTCCAAAAAATCA | ||
| tRWF1 | Park et al. [14] | Forward | AACTAATARCCTTCAAAG | 50 | ± 860 |
| LepR1 | Hebert et al. [13] | Reverse | TAAACTTCTGGATGTCCAAAAAATCA | ||
| LCO1490 | Folmer et al. [11] | Forward | GGTCAACAAATCATAAAGATATTGG | 48 | 658 |
| HCO2198 | Folmer et al. [11] | Reverse | TAAACTTCAGGGTGACCAAAAAATCA |