Fig. 4.

OsJRL45 promotes seed germination and seedling growth of rice under salt stress. A A schematic diagram of CDS coding sequence structure of OsJRL45. Black and gray rectangles boxes respectively represent the exons and intron, and the CRISPR/Cas9 targeting site of the osjrl45 knockout (KO) plants are showed in the figure. The 1 nt insertions in OsJRL45 exons in two knockout mutants are also indicate. The asterisks are used to show the premature stop codon in the deduced amino acid sequence of the mutant OsJRL45 protein. B Transcription levels of OsJRL45 in the leaves of WT and OsJRL45 overexpression plants (OE1-5). C-D Germination test (C), shoot length (D), and root length (E) of WT, KO1, KO2, OE1, and OE2 genotypes grown in different concentrations of NaCl. Seeds were dehulled, and incubated first on Murashige and Skoog (MS)-agar medium for 2 d and then on MS-agar medium containing 100 mM or 200 mM salt for 7 d. Data present mean ± SD of three biological replicates. Asterisks show statistically significant differences (*P < 0.05, **P < 0.01; Student’s t-test)