Skip to main content
. 2023 Oct 25;14:1292839. doi: 10.3389/fimmu.2023.1292839

Figure 4.

Figure 4

HER2-targeted VHH-Fc promotes internalization and mediates growth inhibition in SKBR3 and NCI-N87 tumor cells. (A) HER2 internalization induced by VHH-Fc in comparison with trastuzumab and pertuzumab. SKBR3 cells were incubated with antibodies for 0.5 or 4 hours. The flow cytometry was used to determine the mean percent internalization. Data were mean ± SD (n=3) and two-way ANOVA with Tukey’s multiple comparisons test was performed. The p values were provided in Supplementary Table 1 . (B) VHH-Fc mediated tumor growth inhibition in NCI-N87 cells. The percentage of cell viability was measured by CCK-8 assay. (Up) The representative curve means the concentration-dependent inhibition induced by VHH-Fc. (Down) A2G5-Fc, A9B5-Fc, and H2F5-Fc conferred moderate cell proliferation blocking, but G1E4-Fc showed significant agonistic effect. Data were mean ± SD (n=3) and one-way ANOVA with Dunnett’s multiple comparisons test was performed. The p values were provided in Supplementary Table 2 . *p < 0.0332, ***p < 0.0002, ****p < 0.0001.