Table 2.

The affinities of anti-HER2 nanobodies binding to HER2-ECD after affinity maturation.

	Affinity
Anti-HER2 nanobodies	KD (nM)	kon(1/Ms)	kdis(1/s)
A2H4-H3	42.17	3.16 × 104	1.33 × 10-3
A2H4-G5	14.37	5.11 × 104	7.34 × 10-4
A9H5-F5	19.38	2.76 × 104	5.35 × 10-4
A9H5-B5	7.801	5.93 × 104	4.63 × 10-4
H2C11-A6	7.25	4.59 × 104	3.32 × 10-4
H2C11-F5	3.89	9.87 × 104	3.84 × 10-4
G1H2-H4	88.3	3.82 × 104	3.37 × 10-3
G1H2-E4	53.4	3.64 × 104	1.94 × 10-3