FIG. 3.

lacZ expression of Acinetobacter sp. strain WH435 (ppk::lacZ) grown in minimal medium with different phosphate concentrations. Cultures used for inoculation were grown in minimal medium with 1 mM phosphate. The OD₆₀₀s (closed symbols) and β-galactosidase activities (open symbols) for cultures grown in medium supplemented with 10 μM (▾ and ▿), 100 μM (• and ○), and 1 mM (▴ and ▵) phosphate are shown. The means and standard deviations of three independently grown cultures are shown. Growth of ADP1 was identical to that WH435, but no β-galactosidase activity was demonstrated. Arrows indicate the samples used for preparation of RNA from ADP1 cultures for Northern blot and primer extension.