Fig. 1.

NagC regulates Poly-GlcNAc production on planktonic Y. pseudotuberculosis cells at 37 °C. CLSM revealed that there was less Rho-WGA staining of poly-GlcNAc in planktonic cultures in the Gfp-labelled nagC mutant when compared to the parent at 37 °C. Left panels, Gfp-labelled Y. pseudotuberculosis ; right panels, Rho-WGA staining of poly-GlcNAc (a). When the CLSM images were quantified, a similar pattern emerges with mean fluorescence as a measure of biofilm reduced in the nagC mutant when compared to the parent and complement (b) (n=3 *P=<0.01).