. 2023 Oct 16;5(10):000595.v3. doi: 10.1099/acmi.0.000595.v3

Table 1.

List of primers used for the detection of plasmid mediated colistin resistance

Gene	Primer	Amplicon size	Melting point	Reference
mcr-1	F-CGGTCAGTCCGTTTGTTC	309 bp	58 °C	Ahmed et al. [38]
mcr-1	R-CTTGGTCGGTCTGTAGGG	309 bp	58 °C	Ahmed et al. [38]
mcr-2	F-TGGTACAGCCCCTTTATT	1747 bp	49 °C	Yang et al. [39]
mcr-2	R-GCTTGAGATTGGGTTATGA	1747 bp	49 °C	Yang et al. [39]
mcr-3	F-TTGGCACTGTATTTTGCATTT	542 bp	54 °C	Roer et al. [40]
mcr-3	R-TTAACGAAATTGGCTGGAACA	542 bp	54 °C	Roer et al. [40]
mcr-4	F-ATTGGGATAGTCGCCTTTTT	487 bp	52 °C	Roer et al. [40]
mcr-4	R-TTACAGCCAGAATCATTATCA	487 bp	52 °C	Roer et al. [40]
mcr-5	F-ATGCGGTTGTCTGCATTTATC	1541 bp	50 °C	Borowiak et al. [41]
mcr-5	R-TCATTGTGGTTGTCCTTTTCTG	1541 bp	50 °C	Borowiak et al. [41]