TABLE 1.
Bacterial strains used in this study
| Species | Strain | Source or reference | Reaction with:
|
|
|---|---|---|---|---|
| Universal primersa | Specific primersb | |||
| Butyrivibrio fibrisolvens | C130a, C211, C219 | 2 | + | − |
| Butyrivibrio fibrisolvens | CF3, H17c | M. P. Bryant, University of Illinois | + | − |
| Butyrivibrio fibrisolvens | WV1 | Laboratory strain | + | − |
| Butyrivibrio fibrisolvens | OR509 | R. Teather, Centre for Food and Animal Research, Agriculture and Agri-food, Canada | + | − |
| Butyrivibrio fibrisolvens like | C122a, C21b | 2 | + | − |
| Clostridium aminophilum | F | J. B. Russell, Cornell University | + | − |
| Clostridium clostridioforme | ATCC 25537, ATCC 29084 | ATCCc | + | − |
| Clostridium proteoclasticum | ATCC 51982 | 2 | + | + |
| Clostridium sticklandii | SR | J. B. Russell, Cornell University | + | − |
| Enterococcus faecalis | NCTC 775 | S. Flint, New Zealand Dairy Research Institute | + | − |
| Enterococcus faecalis | 68a | Laboratory strain | + | − |
| Eubacterium cellulosolvens | 5494 | M. P. Bryant | + | − |
| Eubacterium limosum | ATCC 8486 | M. P. Bryant | + | − |
| Eubacterium ruminantium | M. P. Bryant | + | − | |
| Eubacterium sp. | C11, C12b, C13b, C14b#2, C118b, C119b, C120a, C124b, C125b, C260 | 2 | + | − |
| Fibrobacter succinogenes | AC3 | M. P. Bryant | + | − |
| Lachnospira multiparus | ATCC 19307 | M. P. Bryant | + | − |
| Megasphaera elsdenii | T81 | M. P. Bryant | + | − |
| Peptostreptococcus anaerobius | C | J. B. Russell | + | − |
| Peptostreptococcus productus | SF-50 | M. P. Bryant | + | − |
| Prevotella ruminicola | C21a | 2 | + | − |
| Prevotella ruminicola subsp. brevis | 118b, B14 | M. P. Bryant | + | − |
| Prevotella ruminicola subsp. ruminicola | 23 | M. P. Bryant | + | − |
| Ruminococcus albus | 7, 8 | M. P. Bryant | + | − |
| Ruminococcus flavefaciens | ATCC 19208, FD1 | M. P. Bryant | + | − |
| Selenomonas ruminantium subsp. lactilytica | GA31 | M. P. Bryant | + | − |
| Selenomonas ruminantium subsp. ruminantium | ATCC 12561, ATCC 27209 | M. P. Bryant | + | − |
| Streptococcus bovis | JB1 | M. P. Bryant | + | − |
| Streptococcus bovis | NCFB 2476 | A. G. Williams, Hannah Research Institute | + | − |
| Streptococcus bovis | 3-31, 3-36, 3-39, 5-21, 7-2, 7-25, 7-26 | J. B. Russell | + | − |
| Streptococcus bovis | A12, A14, A120, A166, A191b, B11, B32a, B314, B315, B327, B337, B342, B346, B348, B350, B360, B372, B382, B385, B395, B396, B398, C14b#1, C17, C123b, C271 | 2 | + | − |
| Streptococcus bovis | RF-1, RF-2, UDY-KF | Laboratory strains | + | − |
| Succinomonas amylolytica | ATCC 19206 | M. P. Bryant | + | − |
| Succinovibrio dextrinosolvens | ATCC 27209 | M. P. Bryant | + | − |
a
PCR amplification with universal forward and universal reverse primers.
b
PCR amplification with universal forward primer and S-S-Cprot-0832-a-A-21.
c
ATCC, American Type Culture Collection, Rockville, Md.