| Antibodies |
|
| Alexa Fluor 647 anti-mouse CD206 (MMR) Antibody |
BioLegend |
Cat# 141712; RRID: AB_10900420 |
| Alexa Fluor 700 anti-mouse Ly-6C Antibody |
BioLegend |
Cat# 128024: RRID: AB_10643270 |
| Alexa Fluor® 488 anti-mouse Ly-6G Antibody |
Biolegend |
Cat# 127626; RRID: AB_2561340 |
| APC/Cy7 anti-mouse/human CD11b Antibody |
BioLegend |
Cat# 101226; RRID: AB_830642 |
| BD Pharmingen™ APC Rat Anti-Mouse CD19 |
BD Biosciences |
Cat# 550992; RRID: AB_10893995 |
| Brilliant Violet 421™ anti-mouse F4/80 Antibody |
Biolegend |
Cat# 123137; RRID: AB_2563102 |
| Brilliant Violet 510™ anti-mouse I-A/I-E Antibody |
Biolegend |
Cat# 107635; RRID: AB_2561397 |
| Brilliant Violet 711™ anti-mouse CD45 Antibody |
BioLegend |
Cat# 103147; RRID: AB_2564383 |
| Cy5 AffiniPure Donkey Anti-Goat IgG (H + L) |
Jackson ImmunoResearch |
Cat# 705-175-147; RRID: AB_2340415 |
| Donkey anti-Goat IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568, Thermo Fisher Scientific |
Thermo Fisher Scientific |
Cat# A-11057; RRID: AB_2534104 |
| Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488, Thermo Fisher Scientific |
Thermo Fisher Scientific |
Cat# A-21206; RRID: AB_2535792 |
| Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568, Thermo Fisher Scientific |
Thermo Fisher Scientific |
Cat# A10042; RRID: AB_2534017 |
| Donkey Anti-Rat IgG (H + L) Antibody, Alexa Fluor 488 Conjugated, Molecular Probes |
Thermo Fisher Scientific |
Cat# A-21208; RRID: AB_2535794 |
| FITC anti-mouse CD117 (c-Kit) Antibody |
BioLegend |
Cat#105805; RRID: AB_10060183 |
| Goat anti-mouse Iba-1 |
FUJIFILM (Wako) |
Cat# 011-27991; RRID: AB_2935833 |
| Goat Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 647 Conjugate) antibody |
Cell Signaling Technology |
Cat# 4410; RRID: AB_1904023 |
| Goat Anti-Mouse IgG1 (HRP) preadsorbed |
Abcam |
Cat# ab98693; RRID: AB_10674928 |
| Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, HRP |
Thermo Fisher Scientific |
Cat# G-21234; RRID: AB_2536530 |
| Goat anti-Rat IgG (H + L) Cross-Adsorbed Secondary Antibody, HRP |
Thermo Fisher Scientific |
Cat# A18871; RRID: AB_2535648 |
| Human/Mouse CD117/c-kit Antibody |
R&D |
Cat# AF1356; RRID: AB_354750 |
| Lab Vision™ Actin, pan Ab-5, Mouse Monoclonal Antibody |
Fisher Scientific |
Cat# MS-1295-P1; RRID: AB_63316 |
| Mouse monoclonal anti-Aβ1-5, clone 3D6 |
Lilly |
N/A, gift |
| PE anti-mouse FcεRIα Antibody |
BioLegend |
Cat# 134307; RRID: AB_1626104 |
| PE/Cyanine7 anti-mouse CD117 (c-Kit) Antibody |
Biolegend |
Cat# 105813; RRID: AB_313222 |
| PE/Cyanine7 anti-mouse CD19 Antibody |
Biolegend |
Cat# 115520; RRID: AB_313655 |
| PerCP/Cyanine5.5 anti-mouse CD107a (LAMP-1) Antibody |
BioLegend |
Cat# 121626; RRID: AB_2572055 |
| Purified anti-mouse CD16/32 Antibody (clone 93) |
Biolegend |
Cat# 101301; RRID: AB_312800 |
| Purified anti-β-Amyloid, 1–16 Antibody (6E10) |
BioLegend |
Cat# 803001; RRID: AB_2564653 |
| Rabbit anti-APP C terminus (C66) |
In-house |
https://pubmed.ncbi.nlm.nih.gov/34010653/
|
| Rat anti-human/mouse GFAP Monoclonal Antibody (2.2B10) |
Thermo Fisher Scientific |
Cat# 13–0300; RRID: AB_2532994 |
| Recombinant rabbit Anti-Apolipoprotein E (APOE) antibody [EPR19378] |
Abcam |
Cat# ab183596; RRID: AB_2832971 |
| sAPPβ-sw (6A1) Anti-Human Mouse IgG MoAb |
IBL-America |
Cat# JP10321; RRID: AB_1630822 |
|
| Chemicals, peptides, and recombinant proteins |
|
| 2-mercaptoethanol |
Sigma-Aldrich |
Cat# M3148-25ML |
| 10X PBS Solution pH 7.4 |
FISHER |
Cat# P0496 |
| Absolute Ethanol (200 proof) |
Fisher Scientific |
Cat# BP2818-4 |
| Bovine serum albumin (BSA) |
Fisher |
Cat# BP-1600-100 |
| Collagenase D (100 mg) |
Sigma-Aldrich |
Cat# 11088858001 |
| DAPI solution |
Thermo Fisher Scientific |
Cat# 62248 |
| Deoxyribonuclease I |
Worthington |
Cat# LS002004
|
| Dispase II |
Millipore Sigma |
Cat# D4693 |
| EDTA (0.5 M), pH 8.0, RNase-free |
Thermo Fisher Scientific |
Cat# AM9261 |
| Ethylene glycol |
Fisher Scientific |
Cat# BP230-1 |
| FLUKA Formic acid, for mass spectrometry, ~98% |
Thomas Scientific |
Cat# 94318-250ML |
| Glycerol |
Fisher Scientific |
Cat# G31-500 |
| Halt phosphatase inhibitor cocktail |
Thermo Fisher Scientific |
Cat# 1862495 |
| HBSS with calcium and magnesium |
Thermo |
Cat# 24020117 |
| HBSS without calcium and magnesium |
Gibco |
Cat# 14170 |
| HistoPrep Xylene |
Fisher Scientific |
Cat# HC-700-1GAL |
| Hoechst 33342 Solution (20 mM) |
Thermo Fisher Scientific |
Cat# 62249 |
| Hyaluronidase from bovine testes |
Millipore Sigma |
Cat# H3506-100MG |
| M-PER™ Mammalian Protein Extraction Reagent |
Thermo Fisher Scientific |
Cat# 78501 |
| Normal donkey serum |
Sigma-Aldrich |
Cat# D9663, RRID:AB_2810235 |
| OneComp eBeads™ Compensation Beads |
Thermo Fisher Scientific |
Cat# 01-1111-42 |
| Papain from papaya latex |
Sigma-Aldrich |
Cat# P4762-100MG |
| Paraformaldehyde (32%) |
Electron Microscopy Sciences |
Cat# 50-980-495 |
| Percoll |
GE Healthcare |
Cat# 17-0891-01 |
| Pierce ECL Western Blotting Substrate |
Thermo Fisher Scientific |
Cat# 32106 |
| PowerTrack SYBR Green Master Mix |
Thermo Fisher Scientific |
Cat# A46110 |
| ProLong™ Gold Antifade Mountant |
Thermo Fisher Scientific |
Cat# P36934
|
| QIAzol Lysis Reagent |
Qiagen |
Cat# 79306 |
| RBC Lysis Buffer (10X) |
BioLegend |
Cat# 420301 |
| Restore Western Blot Stripping Buffer |
Thermo Fisher Scientific |
Cat# 21059 |
| Richard-Allan Scientific Cytoseal XYL |
Thermo Fisher Scientific |
Cat# 8312-4 |
| RNAprotect Tissue Reagent |
Qiagen |
Cat# 76104 |
| RPMI 1640 Medium, no phenol red |
Thermo Fisher Scientific |
Cat# 11835030 |
| Sodium azide |
Sigma-Aldrich |
Cat# 71289-5G |
| Sodium Chloride (Crystalline/Certified ACS) |
Fisher Scientific |
Cat# S271-3 |
| Sucrose |
Millipore Sigma |
Cat# 84097 |
| SuperBlock™ T20 (TBS) Blocking Buffer |
Thermo Fisher Scientific |
Cat# 37536 |
| SuperSignal West Femto Maximum |
Thermo Fisher Scientific |
Cat# 34096 |
| Sensitivity Substrate |
|
|
| TBS, Tris-Buffered Saline, 10X Solution, pH 7.4 |
Fisher Scientific |
Cat# BP24711 |
| Toluidine blue dye |
Sigma-Aldrich |
Cat#198161-5G |
| Triton X-100 |
Sigma-Aldrich |
Cat# TX1568-1 |
| Xpert Prestained Protein Marker |
GenDEPOT |
Cat# P8502-050 |
|
| Critical commercial assays |
|
| Human/rat beta amyloid (40) ELISA Kit |
Wako |
Cat# 294-62501 |
| Human/rat beta amyloid (42) ELISA Kit |
Wako |
Cat# 290-62601 |
| LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit, for 405 nm excitation |
Thermo Fisher Scientific |
Cat# L34968
|
| Pierce BCA Protein Assay Kit |
Thermo Fisher Scientific |
Cat# 23225 |
| RNeasy Lipid Tissue Mini Kit |
Qiagen |
Cat# 74804 |
| RNeasy Plus Mini Kit |
Qiagen |
Cat# 74134 |
| SuperScript™ IV VILO™ Master Mix with ezDNase™ Enzyme |
Thermo Fisher Scientific |
Cat# 11766050 |
| V-PLEX Proinflammatory Panel1 (mouse) Kit (5 Plate) |
Meso Scale Diagnostics |
K15048D-2 |
|
| Deposited data |
|
| Raw sequencing data files for single-cell RNA sequencing |
This paper |
GEO accession: GSE240687
|
|
| Experimental models: Organisms/strains |
|
| 5XFAD and WT Mice |
The Jackson Laboratory |
Cat# 034848-JAX, RRID:MMRRC_034848-JAX |
| C57BL/6J-KitW-v/J Mice |
The Jackson Laboratory |
Cat# JAX:000049, RRID:IMSR_JAX:000049 |
| CreMaster Mice |
Rodewald Lab |
N/A |
|
| Software and algorithms |
|
| Adobe Illustrator (version 25.3.1) |
Adobe |
RRID:SCR_010279 |
| EthoVision XT (version 9.0) |
Noldus Information Technology |
RRID:SCR_000441 |
| FACSDiva Software (version 6.1.3) |
BD Biosciences |
RRID:SCR_001456 |
| FIJI-ImageJ |
NIH |
RRID:SCR_003070 |
| FlowJo (version 10.7.1) |
Tree Star |
RRID:SCR_008520 |
| GraphPad Prism 7 and 8 |
GraphPad Software |
RRID:SCR_002798 |
| NIS-Elements Advanced Research software (version 5.42.01) |
Nikon |
https://www.microscope.healthcare.nikon.com
|
