Table 1.

Summary of different DCV cargo properties

Cargo	Persistent events (%)	kDa*	Stimulation	Study
SemapHluorin	36 ± 6	90–130	60 mm KCl	De Wit et al. (2009)
SemapHluorin	38.2 ± 6.8	90–130	16 × 50 APs at 50 Hz	De Wit et al. (2009)
BDNF-pHluorin	26.5 ± 7.2	58–42	60 mm KCl	De Wit et al. (2009)
tPA-SpH	22 ± 5	97	60 mm KCl	De Wit et al. (2009)
NPY-pHluorin	7 ± 3	34–42	60 mm KCl	De Wit et al. (2009)
SemaΔBD-pHluorin#	5.8 ± 5.2	80–120	60 mm KCl	De Wit et al. (2009)
NPY-pHluorin	7.7 (median)	34–42	16 × 50 APs at 50 Hz	This study, Figure 2
NPY-pHluorin	0 (median)	34–42	TBS	This study, Figure 2
BDNF-pHluorin	60.75 (median)	58–42	16 × 50 APs at 50 Hz	This study, Figure 2
BDNF-pHluorin	46.3 (median)	58–42	TBS	This study, Figure 2

De Wit et al. (2009).

*kDa depends on degree of peptide processing.

^#SemaΔBD-pHluorin: Semaphorin3A lacking C-terminal polybasic domain that interacts with luminal glycoproteins.

Persistent events are characterized by an abrupt increase in fluorescence that remained elevated above baseline (30 s in 80% of events).

Persistent events are plotted as mean ± SEM.

TBS consists of 5 bursts of 4 APs at 100Hz with 200-ms interval repeated three to four times.