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[Preprint]. 2023 Oct 23:2023.10.20.562785. [Version 1] doi: 10.1101/2023.10.20.562785

Figure 5. LC-MOR receptor rescue reverses neuropathic injury-induced hypersensitivity.

Figure 5.

(A) Cartoon of hMOR viral strategy with immunohistochemical validation of viral expression. Tyrosine Hydroxylase=TH (green), hMOR-expressing neurons are filled with oScarlet (red). (B) Experimental timeline. (C&D) Cell-attached ex vivo electrophysiology of DAMGO-mediated suppression of LC firing in Oprm1fl/flxDbhCre+/− mice without hMOR (C) and with hMOR expression (D). (E) Quantification shows significant rescue of DAMGO-induced inhibitory responses in the LC of Oprm1fl/flxDbhCre+/− mice with hMOR compared to those without. Data expressed as mean +/− SEM, n=9–16/group, two-way ANOVA with Tukey’s multiple comparisons *p<0.05. (F) Cartoon describing spared nerve injury model. (G) Rescue of MOR expression reverses neuropathic pain-induced thermal hypersensitivity. Data expressed as mean +/− SEM, n=8–10/group, two-way ANOVA Cre+ injured paw 8 weeks vs. hMOR **p<0.01. (H) Rescue of MOR expression reverses neuropathic pain-induced mechanical hypersensitivity. Data expressed as mean +/− SEM, n=8–10/group, two-way ANOVA Cre+ injured paw 8 weeks vs. hMOR *p<0.05.